ABSTRACT
OBJECTIVE:To establish a method for the content determination of 1-phenyl-7-(3-methoxy-4-hydroxy) phe-nyl-5-ol-3-heptanone in active ingredients of Mongolian medicine compound shudage-4. METHODS:HPLC was performed on the column of Intersil ODS-3 with the mobile phase of acetonitrile-0.5%phosphoric acid at the flow rate of 1.0 ml/min,the detection wavelength was 210 nm,temperature was 30 ℃,and the volume was 20 μl. RESULTS:The linear range of 1-phenyl-7-(3-me-thoxy-4-hydroxy) phenyl-5-ol-3-heptanone was 0.102-1.02 μg(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 1.52%;average recovery was 97.10%(RSD=1.80%,n=6). CONCLUSIONS:The method is accurate,sim-ple and reproducible,and can provide basis for the quality control of Mongolian medicine compound shudage-4.
ABSTRACT
OBJECTIVE:To establish a method for the determining contents of 2 lignan components[dehydrodiisoeugenol and 2,3-dihydro-7-methoxy-2-(3,4-methylened ioxyphenyl)-3-methyl-5-(E)-propenyl-benzofuran(referred to“lignanoid 2”)]. METH-ODS:HPLC method was adopted. The column was Elite C18 with the mobile phase of water-methanol(gradient elution)at the flow rate of 1.0 ml/min;the detection wavelength was 225 nm and the column temperature was 30 ℃. The sample size was 20 μl. RE-SULTS:There was a good linear relationship between sample quantity and the peak area in the range of 0.202-2.02 μg(r=0.999 9) and 0.204-2.04 μg(r=0.999 9)for 2 lignan components. The RSD of precision,stability and repeatability tests were less than 2%with the average recovery of 101.54%(RSD=0.60%,n=6)and 99.43%(RSD=1.09%,n=6). CONCLUSIONS:The method is simple,sensitive and accurate,and can be used for the quantization determination of dehydrodiisoeugenol and lignanoid 2 in nut-meg-5.